FeFAB cells (a kind gift from fefabDr Martin Löchelt, Germany) are modified CRFK cells that are used for FFV infection experiments. FeFAB cells express β-galactosidase under the control of the FFV LTR, which is trans。
Briefly, FeFAB cells are the modified CRFK cells that express β-galactosidfefabase under the control of the FFV LTR, which is trans-activated by the FFV Tas protein in relation to。
HEK293T cfefabells were cotransfected with pCF-BBtr, the empty vector pcDNA, or pcfeA3Z2b-HA, and increasing amounts of plasmids encoding either wt or mutant Bet (Figure。
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